Изучение реакции цитохрома bd‑I Escherichia coli в полностью восстановленном состоянии с цианидом с использованием спектроскопии поглощения и кругового дихроизма

Аннотация

Ранее мы начали исследование реакции изолированного солюбилизированного цитохрома bd‑I Escherichia coli, восстановленного дитионитом, с цианидом [Borisov и Arutyunyan (2024) J. Inorg. Biochem., 259, 112653]. Настоящая работа является продолжением этого исследования. С помощью методов спектроскопии поглощения и КД получены следующие новые результаты. 1) Мембранная форма полностью восстановленного (FR) фермента также способна связывать цианид. Значения кажущейся константы диссоциации и константы скорости второго порядка составляют 81,1 ± 7,8 мМ KCN и 0,11 ± 0,01 M⁻¹∙с⁻¹ соответственно. Это противоречит данным работ других исследователей, согласно которым оксидаза bd‑I, находящаяся в нативных мембранах, в FR‑состоянии не связывает цианид. 2) СО, добавленная к циано-аддукту как мембранной, так и изолированной солюбилизированной формы FR‑цитохрома bd‑I, вытесняет цианид, приводя к образованию комплекса фермента с СО. Это свидетельствует об обратимости связывания цианида с белком. Для насыщения сайта связывания оксидазы CO в присутствии 100 мМ KCN требуется гораздо больше CO, чем в случае добавления CO к необработанному цианидом ферменту. СО и цианид конкурируют между собой за связывание с одним и тем же сайтом в оксидазе – гемом d²⁺, причём СО, являясь более сильным лигандом, такую конкуренцию у цианида выигрывает. 3) Исследовано влияние цианида на оптическую активность FR‑цитохрома bd‑I. Спектры КД FR‑фермента, полученные до и после обработки цианидом, свидетельствуют о том, что образование циано-аддукта гема d²⁺ приводит к значительному ослаблению экситонных взаимодействий между гемом d²⁺ и гемом b₅₉₅²⁺. Предложены схемы взаимодействия цианида и СО в присутствии избытка цианида с активным центром FR‑фермента.

Сноски

* Адресат для корреспонденции.

Вклад авторов

В.Б. Борисов – концепция и руководство работой; В.Б. Борисов, А.М. Арутюнян – проведение экспериментов, обработка и обсуждение результатов исследования, написание и редактирование текста статьи.

Финансирование

Исследование выполнено за счёт гранта Российского научного фонда № 24-24-00006 (https://rscf.ru/project/24-24-00006/).

Благодарности

Авторы выражают благодарность Р.Б. Геннису (Иллинойский Университет, Урбана, Иллинойс, США) за штамм E. coli GO105/pTK1.

Конфликт интересов

Авторы заявляют об отсутствии конфликта интересов в финансовой или какой-либо другой сфере.

Соблюдение этических норм

Эта статья не содержит никаких исследований с участием людей или животных, проведённых кем-либо из авторов.

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