Сверхспирализация ДНК снижает холодочувствительность плавления промотора РНК‑полимеразами Deinococcus-Thermus

Аннотация

Плавление промоторной ДНК вокруг стартовой точки транскрипции является важнейшим этапом транскрипции, необходимым для инициации синтеза РНК. У бактерий плавление промотора осуществляется холоферментом РНК‑полимеразы (РНКП), состоящим из каталитического кор‑фермента и σ‑фактора, участвующего в распознавании промотора. Ранее было обнаружено, что РНКП из термофильной бактерии Thermus aquaticus и мезофильной бактерии Deinococcus radiodurans не способны инициировать транскрипцию при низкой или умеренной температуре. Эти свойства зависят от их σ‑факторов, а гибридные холоферменты, включающие эти σ‑факторы и кор‑фермент Escherichia coli, обладают похожими свойствами. В данной работе показано, что сверхспирализация ДНК снижает холодочувствительность плавления промотора РНКП Deinococcus–Thermus и гибридными холоферментами. Сверхспирализация также повышает стабильность промоторных комплексов этих РНКП и снижает их чувствительность к высокой ионной силе. Подобные свойства РНКП Deinococcus-Thermus позволяют предположить, что их активность может регулироваться степенью сверхспирализации генома, в том числе при стрессовом ответе клеток.

Финансирование

Работа частично выполнена в рамках государственного задания НИЦ «Курчатовский институт» и ИБГ РАН.

Конфликт интересов

Автор заявляет об отсутствии конфликта интересов.

Соблюдение этических норм

Настоящая статья не содержит описания каких-либо исследований с участием людей или животных в качестве объектов.

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