Конъюгаты десмикозина с фрагментами антимикробного пептида онкоцина: синтез, антибактериальная активность, взаимодействие с рибосомой

Сноски

* Адресат для корреспонденции.

Финансирование

Работа выполнена при финансовой поддержке Российского фонда фундаментальных исследований (проекты № 20-04-00873-a (синтез аналогов, молекулярное моделирование, связывание с рибосомами), № 19-14-00115 (ЯМР исследования) и № 21-64-00006 (in vitro трансляция, антибактериальная активность)).

Благодарности

Мы благодарим А.А. Богданова за инициирование и поддержку работ по изучению пептидных производных рибосомных антибиотиков. Авторы также благодарят А.Л. Коневегу за предоставленные для работы рибосомы; М.В. Серебрякову за масс-спектрометрические анализы; А.Л. Ксенофонтова за проведение аминокислотного анализа; О.Ю. Савельева за экспертную техническую помощь в измерениях ЯМР. Работа выполнена с использованием оборудования, приобретённого за счёт средств Программы развития Московского университета. Авторы выражают благодарность МГУ имени М.В. Ломоносова за возможность использовать установки ЯМР, а также Вычислительному центру МГУ имени М.В. Ломоносова за предоставленную возможность вести расчёты молекулярной динамики на суперкомпьютере «Ломоносов-II».

Вклад авторов

З.З. Хайруллина – проведение экспериментов (синтез, связывание с рибосомами), обсуждение результатов исследования, написание текста, редактирование текста; Г.И. Макаров – моделирование молекулярной динамики, обсуждение результатов исследования, написание и редактирование текста; А.Г. Терещенков – проведение экспериментов (связывание с рибосомами), обсуждение результатов, написание и редактирование текста, оформление рисунков; В.С. Буев – проведение экспериментов (in vitro трансляция, антибактериальная активность); Д.А. Лукьянов – проведение экспериментов (in vitro трансляция, антибактериальная активность); В.И. Польшаков – проведение экспериментов (ЯМР), анализ данных; В.Н. Ташлицкий – проведение экспериментов (хроматография, масс-спектрометрия); И.А. Остерман – руководство работой, обсуждение результатов исследования; Н.В. Сумбатян – концепция и руководство работой, обсуждение результатов, написание и редактирование текста.

Конфликт интересов

Авторы заявляют об отсутствии конфликта интересов.

Соблюдение этических норм

Настоящая статья не содержит описания каких-либо исследований с участием людей или животных в качестве объектов.

Дополнительные материалы

Дополнительные материалы

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