БИОХИМИЯ, 2019, том 84, вып. 10, с. 1460–1472

УДК 612.01.8;612.017.1;611.018.53

Роль каспаз в проявлении цитотоксичности клеток NK-92 в различных моделях их сокультивирования с клетками трофобласта*

© 2019 Ю.П. Милютина **, В.А. Михайлова, К.М. Пятыгина, Е.С. Демидова, Д.А. Малыгина, Т.Е. Тертычная, А.В. Арутюнян, Д.И. Соколов, С.А. Сельков

Федеральное государственное бюджетное учреждение «НИИ акушерства и гинекологии им. Д.О. Отта» Северо-Западного отделения РАМН, 199034 Санкт-Петербург, Россия; электронная почта: milyutina1010@mail.ru

Поступила в редакцию 17.04.2019
После доработки 18.06.2019
Принята к публикации 22.06.2019

DOI: 10.1134/S0320972519100087

КЛЮЧЕВЫЕ СЛОВА: каспазы, гранзим B, трофобласт, естественные киллеры, апоптоз.

Аннотация

Изучение особенностей взаимодействия естественных киллеров с клетками трофобласта и выявление условий, при которых NK-клетки способны реализовывать свою цитотоксическую функцию, имеет важное фундаментальное и прикладное значение для понимания их роли в развитии патологических процессов и осложнений в период беременности. В данной работе продемонстрированы особенности изменения содержания и активации каспаз в клетках трофобласта линии Jeg-3 в различных моделях их сокультивирования с клетками линии NK-92 и показана необходимость непосредственного контакта между клетками данных популяций для активации каспазы-8 и каспазы-3 в клетках трофобласта. В результате взаимодействия в клетках линии Jeg-3 обнаруживается цитотоксический белок гранзим B, что сопровождается снижением его количества в клетках линии NK-92. Дистантное сокультивирование клеток линий NK-92 и Jeg-3 не приводит к активации инициаторной и эффекторной каспаз в клетках линии Jeg-3, характерной для картины развития апоптоза. При этом снижение исследуемых прокаспаз в клетках трофобласта может быть обусловлено реализацией их альтернативных неапоптических функций.

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Сноски

* Первоначально английский вариант рукописи опубликован на сайте «Biochemistry» (Moscow) http://protein.bio.msu.ru/biokhimiya, в рубрике «Papers in Press», BM19-121, 16.09.2019.

** Адресат для корреспонденции.

Финансирование

Выполнено при финансовой поддержке НШ-2873.2018.7 и Госзадания АААА-А19-119021290116-1.

Конфликт интересов

Авторы заявляют об отсутствии конфликта интересов.

Соблюдение этических норм

Настоящая статья не содержит описания каких-либо исследований с использованием людей или животных в качестве объектов.

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