БИОХИМИЯ, 2019, том 84, вып. 4, с. 571–579

УДК 577.214

σ24-Субъединица РНК-полимеразы Escherichia coli способна вызывать паузы транскрипции in vitro*

© 2019 А.Б. Шикалов, Д.М. Есюнина, Д.В. Пупов, А.В. Кульбачинский, И.В. Петушков **

Институт молекулярной генетики Российской академии наук, 123182 Москва, Россия; электронная почта: telomer1@rambler.ru

Поступила в редакцию 02.11.2018
После доработки 05.12.2018
Принята к публикации 12.12.2018

DOI: 10.1134/S0320972519040109

КЛЮЧЕВЫЕ СЛОВА: паузы транскрипции, РНК-полимераза, альтернативные σ-факторы, Gre-белки.

Аннотация

Бактерия Escherichia coli имеет семь σ-факторов, которые связываются с кор-ферментом РНК-полимеразы (РНКП) и обеспечивают узнавание разных групп промоторов. Ранее было показано, что σ70 и σ38-субъединицы могут также взаимодействовать с элонгационным комплексом (ЭК) и вызывать паузы транскрипции, узнавая последовательности ДНК, которые напоминают –10-элемент промотора. В данной работе исследована способность σ32, σ28 и σ24-субъединиц вызывать паузы транскрипции in vitro в реконструированных ЭК, содержащих соответствующие консенсусные –10-элементы. Показано, что σ24-субъединица способна вызывать паузу транскрипции в зависимости от наличия –10-элемента. Формирование паузы подавляется Gre-факторами транскрипции. Обнаружено, что некоторые природные промоторы содержат потенциальные сигналы σ24-зависимых пауз в начале транскрибируемой области. Это позволяет предположить, что такие паузы могут играть регуляторную роль в транскрипции.

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Сноски

* Первоначально английский вариант рукописи опубликован на сайте «Biochemistry» (Moscow) http://protein.bio.msu.ru/biokhimiya, в рубрике «Papers in Press», BM 18-301, 11.02.2019.

** Адресат для корреспонденции.

Финансирование

Работа выполнена при финансовой поддержке РНФ (грант 16-14-10377).

Благодарности

Авторы благодарят И. Арцимович за предоставленную плазмиду (pVS10), А. Огиенко за тестирование активности σ28-субъединицы, а также рецензентов за ценные замечания по редактированию рукописи.

Конфликт интересов

Авторы заявляют об отсутствии конфликта по вопросам финансирования и другим вопросам при исполнении данной работы.

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